Meiji Techno MT5000 Series Manual de usuario descargar pdf (Pagina 12)

3.2

ADJUSTING FOR KOEHLER ILLUMINATION

Koehler illumination is

procedure

for

setting up and adiusting

your

microscope

to achieve the

best

possible

combination of contrast and resolution.

1. Turn

the microscope illuminator

and

place

the specimen slide

you

wish to

examine on the

microscope stage. Rotate the 10X

objective

into

position

and

focus

on image of

your

specimen.

2. Move the sub-stage condenser up to its top

position

using the

rack

and

pinion

focusing

control.

3. Check

to make

sure

that

both

the field iris

(on

the

lamp

house) and the aperture iris

(in

the sub-stage

condenser) are fully open. Rotate the Jield iris

control

ring

unlil the edge ol the field iris is

just

visible

at the outer edge ol the

field

oJ view.

Using the

rack

and

pinion

focus control, raise or lower the

sub-stage condenser until the edge

the

lield iris comes into

sharo

{ocus.

the

image

of the

field iris

appears to be off-center in relation to

your

field

view,

then it will be

necessary to

align

the field iris

that it is

centered

the field of view. Rotating the

substage

condenser centering screws in either diection allows

you

to move the image

ol the field iris until it is

concenlric with the field

view.

Field

view

before Condenser

alignment

Field

view

after Condenser

alignment

Field

iris

partially

closed

Field iris

fully

open

With

the

image

of the

field iris

centered and focused as sharply

possible,

open the field iris

diaphragm until the image of the

edge of

the iris

diaphragm

just

outside of the field

view.

7. Remove

one oJ

the

eyepieces and

look into

the eyetube. Observe the disc

light

coming

from

the

back

image

plane

of the

objective. Close down

the

aperture

iris

using the aperture iris lever located

on the side of the sub-stage condenser until approximately two thirds

of the disc of light

you

are

observing

remains visible. Replace the

eyepiece.

The microscope is now

adjusted

for koehler illumination

for

use

with

the

10X

objective.

This

procedure

should

be followed for

each objective

guarantee

critical illumination.

1 2 ... 7 8 9 10 11 12 13 14 15 16 17 ... 27 28

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Meiji Techno MT5000 Series Manual de usuario Pagina 12

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