
-
5. Select a lower
power "scanning"
objective
like the
5X
or
the
10X
to
find the
area
of
interest
on
the
specimen
quickly.
Be sure
the
objective
"clicks"
into
place
when
you
turn
the
objective nosepiece.
6.
By
using the
coarse and fine
focus knobs
shown
above, adjust
your
specimen into
focus
with
your
left
eye
closed
(looking
only
through the right
eyetube).
With
the specimen
in focus,
close
the right
eye
you
just
used and
open the left. lf
the
specimen
is
out
of
focus for the
left eye,
adjust the
diopter
on
the
eyetube until the focus in
correct.
The
microscope is
now
adjusted for this
user. Focusing
eyetubes
allows
us
to compensate
for
users that
wear
corrective eye lenses.
lf
you
wear
glasses,
you
may want
to remove the
rubber
eyeguards.
7. The
aperture
iris
diaphragm
located
on the vertical
illuminator
can be
"stopped
down"
or
closed
somewhat
to
give
the observation
of
your
specimen more
or
less
contrast
or resolving
power.
Stopping
down the diaphragm
decreases
resolution
and
brightness
but
increases
image
contrast and
depth of
focus.
3.3
lncident
Light
Operation
-
Darkfield
1. To
engage
darkfield
mode,
pull
the
darkfield selector lever
until it
comes out with
the darkfield
stopper
in
position
(maximum
right
hand
position).
Be sure
that
both
the Field
lris
and the Aperture
lris
are
opened
completely.
2.Looking into
the
eyepieces without
a specimen on the stage, the
field
of view
appears dark.
3.When a specimen
is
placed
on the stage, features
of the
specimen
such as
scratches,
surface
particles
and depressions
will reflect
brightly
against the
dark
background.
4.Darkfield
mode is
useful for
observing
specimens with features
thal
cannol be readily
seen
under
brightfield
mode.
Possible Darkfield
Mode
Operational Problems
lf normal
adjustments
are not
getting
the results
you
expect,
check to
see if these
conditions
exist:
o
lncorrect
components inadvertently
installed
o
Components not mounted
flush
(Vertical
llluminator
to
microscope frame)
o
Dirty
or
smudged
optics
o
Aperture lris
or Field lris
are completely
closed
il
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