
3.1 Basic Set Up For Transmitted Light – Brightfield
1. First, set your interpupillary distance on the
microscope eyetubes by pulling them apart
or pushing them closer together to fit your
eyes. When set correctly, one will see one
uniform round field of view. Make note of
the distance setting when adjusted to your
liking so you can later repeat the setting.
2. Turn on the microscope power switch as
shown in Figure 1a marked number 10.
Adjust the brightness desired with the
variable brightness control knob shown as
number 8 in Figure 1a.
3. Make sure the aperture iris diaphragm lever
is all the way to the left (maximum opened)
to start. Refer to Figure 1B.
4. We recommend to initially us a specimen
that has areas of high and low contrast.
Place a specimen on the stage.
5. Select a lower power “scanning” objective
like the 4X or the 10X to find the area of interest on the specimen quickly. Be
sure the objective “clicks” into place when you turn the objective nosepiece.
6. By using the course and fine focus knobs shown in Figure 1a, adjust your
specimen into focus with your left eye closed (looking only through the right
eyetube). With the specimen in focus, close the right eye you just used and open
the left. If the specimen is out of focus for the left eye, adjust the diopter on the
eyetube until the focus in correct. The microscope is now adjusted for this user.
Focusing eyetubes allows us to compensate for users that wear corrective eye
lenses. If you wear glasses, you may want to remove the rubber eyeguards. Never
turn the left and right hand focus knobs in the opposite direction as damage to
the mechanism may result.
7. The aperture iris diaphragm located above the condenser and below the integrated
illuminator can be "stopped down" or closed somewhat to give the observation of
your specimen more contrast or resolving power. Stopping down the diaphragm
decreases resolution and brightness but increase image contrast and depth of
focus.
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