Meiji Techno TC5000 Series Manual de instrucciones descargar pdf (Pagina 24)

Possible Phase Contrast Mode Operational Problems

If normal adjustments are not getting the results you expect, check these:

• Wrong phase annulus being used.

• Phase annulus not centered correctly.

• Wrong condenser installed.

• Aperture diaphragm is completely closed.

• Flask or vessel glass too thick on bottom side.

• Halos around the outlines of details are optical artifacts which may obscure details

of your specimen. This is a known limitation of phase contrast microscopy.

• Since phase annuli limit the numerical aperture of the optical system, image

resolution may suffer somewhat.

• If the specimen being observed is too thick, phase shifting will distort the image

details.

TC-5300 and TC-5400 Phase Contrast Objectives

Phase Contrast Objectives - Planachromat - Infinity Corrected - F = 200mm

MA825

TC Planachromat Phase 4X objective ∞/1.0, NA: 0.13, WD = 17.3mm, F.L.= 50.0 (optional)

MA826

TC Planachromat Phase 10X objective ∞/1.0, NA: 0.25, WD = 7.6mm, F.L.= 20.0 (included)

MA827

TC Planachromat Phase 20X objective ∞/1.0, NA: 0.40, WD = 7.0mm, F.L.= 10.0 (included)

MA828

TC Planachromat Phase 40X objective ∞/1.0, NA: 0.65, WD = 2.8mm, F.L.= 5.0 (optional)

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Meiji Techno TC5000 Series Manual de instrucciones Pagina 24